Issue 2

Effects of Directive 2010/63/EU on research on wild-sourced animals: a review of publication trends in studies on captive wild mice (Apodemus sp.)   
Maílis Carrilho, Sophie von Merten, Maria da Luz Mathias and Joaquim T. Tapisso
pp. 145

Decision trees for determining the fate of laboratory animals
Myhailo Reshetnykov, Karen Stumm, Michael Zwick and Jan Baumgart
pp. 157

Mouse aversion to induction with isoflurane using the drop method
Maya J. Bodnar, I. Joanna Makowska, Courtney T. Boyd, Catherine A. Schuppli and Daniel M. Weary
pp. 169

Comparison of inferior vena cava puncture under continuous cardiac perfusion with cardiac puncture in blood acquisition of the laboratory mouse
Wengang Hu, Hao Sheng, JiaCai Yang, Cheng Chen, Ruoyu Shang, Zhihui Liu, Xiaohong Hu, Xiaorong Zhang, Weifeng He, Chibing Huang and Gaoxing Luo
pp. 178

Mouse strain-specific habituation to oral metamizole administration
Tim Schreiber, Emily Leitner, Jakob Brandstetter, Anna Richter, Sandra Lange, Dietmar Zechner, Christian Junghanss, Brigitte Vollmar and Simone Kumstel
pp. 192

Pre-immunization of diet-induced obese male mice with inactivated pathogens increases power in a liraglutide intervention study
Caroline Falkenberg, Dorte B Sørensen, Camilla HF Hansen, Martin F Toft and Axel K Hansen
pp. 203

Jacketed telemetry in rats: a novel non-invasive method for cardiorespiratory phenotyping during treadmill exercise        
Stéphane Tanguy, Agathe Cambier, Leandro Fontana-Pires, Timothé Flenet, Charles Eynard, Julie Fontecave-Jalon, Pierre-Yves Gumery and Franc¸ois Boucher
pp. 215

An adaptable, user-friendly score sheet to monitor welfare in experimental fish
Mathilde Flueck-Giraud, Heike Schmidt-Posthaus, Alessandra Bergadano and Irene Adrian-Kalchhauser
pp. 226

Cardiorespiratory, hemodynamic, and sedative effects of dexmedetomidine in sheep
Janainne Hilbig Gomes, Isabela Plazza Bittar, Rauane Sousa de Moura, Bruna Ginú Prado and Leandro Guimarães Franco
pp. 242

Pitfalls in the analysis conditions of canine brain perfusion computed tomography
Kazuya Kushida, Kodai Tashiro, Masaaki Katayama, Ryuji Fukushima and Miori Kishimoto
pp. 253

The effectiveness of educational workshops in improving the knowledge and attitude of attendees in laboratory animal science: A pre- and post-intervention design in Iran
Siavash Ahmadi-Noorbakhsh, Jila Sadighi, Zahra Hatami and Ehsan Shamsi Gooshki
pp. 261

Preceding sevoflurane inhalation in rats reduces the stress of intraperitoneal injection both in inexperienced experimenters and subject rats
Hidetoshi Ishibashi and Hideyuki Motohashi
pp. 272

Animal researchers’ views on the publication of negative results and subsequent policy adoptions
Carlos Oscar S Sorzano, Daniel G Hamilton and Eva Mendez
pp. 276

Extended oxygen supplementation after thoracotomy in rats may improve welfare      
Bryony Few and Alex Dugdale
pp. 283

Spontaneous hepatocellular and gastric tumours in African clawed frogs (Xenopus laevis)        
Bernat Martí-García, Simon L Priestnall, Yolanda Saavedra Torres and Alejandro Suárez-Bonnet
pp. 290

Comment on Frommlet and Heinze (2021): Correcting the error degrees of freedom 
Reid D. Landes
pp. 299

Response to Landes: Comment on Frommlet and Heinze (2021): correcting the error degrees of freedom      
Georg Heinze and Florian Frommlet
pp. 303