"Too many pre-clinical experiments are giving results which cannot be reproduced. This may be because the experiments are incorrectly designed. In “Completely randomized” (CR) and “Randomised block” (RB) experimental designs, both the assignment of treatments to experimental subjects and the order in which the experiment is done, are randomly determined. These designs have been used successfully in agricultural and industrial research and in clinical trials for nearly a century without excessive levels of irreproducibility. They must also be used in pre-clinical research if the excessive level of irreproducibility is to be eliminated.A survey of 100 papers involving mice and rats was used to determine whether scientists had used the CR or RB designs. The papers were assigned to three categories “Design acceptable”, “Randomised to treatment groups”, so of doubtful validity, or “Room for improvement”. Only 32±4.7% of the papers fell into the first group, although none of them actually named either the CR or RB design.If the current high level of irreproducibility is to be eliminated, it is essential that scientists engaged in pre-clinical research use “Completely randomised” (CR), “Randomised block” (RB), or one of the more specialised named experimental designs described in textbooks on the subject."
The purpose of my Ph.D.-project is to investigate mechanisms involved in chronic pain. I focus on a cell type that under normal conditions support our pain-sensing nerve cells but are proposed to contribute to the painful sensation that follows physical injury or chemotherapy treatment. By investigating the response in this cell type following physical injury or chemotherapy, we will come one step closer to understanding how nerve cells in some cases are affected to signal more intense or chronic pain. Animal Experiments are essential for investigating my questions at hand, and often require a surgical procedure on the sciatic nerve to evoke neuropathic pain in the laboratory animal. When I perform this procedure, it is crucial that I excel, to minimize suffering in the experimental animal. Therefore, I apply for funding to participate in Ph.D. courses to develop my skills in experimental surgery.
This project consists of shooting a science divulgation video titled “En ciencia todo suma” (“In Science Everything Counts”) which will be distributed among all our association members by all channels available: SECAL’s web site, email distribution list, YouTube, Twitter, etc. This video will show the human side of our career together with a scientific message to society aiming to provide why is animal experimentation needed. It will be realistic, hilarious, interactive and committed to Animal Welfare and Science. This video will show caretakers, technicians and investigators together with written material that reveal in an entertaining way our human side.
ExpoBiteriosVirtual (EBV) will be a 100% virtual event, hosted on a novel platform that will enhance the interaction of the attendees with the speakers. The latest technological advances will be displayed at a trade show. The scheduled days for the event will be October 16 and 17, 2020. The virtual experience will have a personalized space for the participants. They will be able to experience the energy of entering an event and seeing all the possibilities of learning and networking. The presentations will be in spanish, portuguese and english (with spanish subtitles). These talks will be available on-demand for the attendees. Registration for EBV has a cost of US $ 25. However, we will provide scholarships for all those who need support.
I am requesting funding for an open source book publication: Experimental Design and Reproducibility in Preclinical Studies. This is part of an Open Source Book Series in Laboratory Animal Science and Medicine. The purpose of the Open Source Book Series is to promote best practice within the field of Laboratory Animal Science and Medicine, and to share worldwide this information so scientists, students and technical staff working in countries with difficulties accessing this type of publications can have an open source reference. This series aim to have a number of published books related to the field of laboratory animal science and medicine with a very practical approach so to facilitate the day-to-day work of the scientists, students and technical staff working with laboratory animals.
SECAL is organizing the IX Annual Scientific Meeting in conjunction with the SECAL Annual General Assembly. The event is totally free and open to everyone, SECAL members and non-members. The topics of the meeting include three different fields: - Euthanasia- new recommendations for mice - Imaging technique and advances in the Three Rs - Facial expression on rodentsDue to the current coronavirus COVID 19 crisis, the topics could be changed/updated and one seminar and a round table about the plans of contingency for animal facilities and the Covid19 pandemic could be also included. This point will be decided at the SECAL Board meeting in May 2020. The funding will be used to several activities: 1. Recording the event in video. 2. Streaming online service. 3. Speakers. Recognized Spanish experts.